[Histonet] How to keep paraffin sections from sticking to blade/blade holder.

[Jennifer MacDonald]

Another culprit is static.  Moisture helps to keep the static down.




"Clough, Bret" <Clough <@t> medicine.tamhsc.edu> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
04/21/2011 12:11 PM

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[Histonet] How to keep paraffin sections from sticking to blade/blade 
holder.






While sectioning paraffin embedded tissue on a rotary  microtome I noticed 
the sections sticking to the blade holder and bunching up. What do I need 
to do so that my section come off the blade in a ribbon? Please note that 
this is my first time in sectioning tissue and this is a learning exercise 
 for me.

Thanks in advance for your suggestions .

Bret
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