[Histonet] decalcification of articular cartilage

[Liz Chlipala]

We do a lot of IHC staining on bone and cartilage samples and we use 10% formic acid for decalcification for immunohistochemistry.  I'm afraid you may not be able to turnaround this in a week.  The tissue needs to be adequately fixed prior to decalcification, and properly decaled.  I would trim the tissue to about 4 mm thick if you can on a saw you might be able to turn this around in  your time frame, but I have learned from experience if you rush decalcification you have a greater chance of running into problems.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nejat Yilmaz
Sent: Wednesday, April 20, 2011 8:12 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] decalcification of articular cartilage

Dear Members,

We're planning to study sheep knee articular cartilage with histochemistry and immunohistchemistry. Proximal parts of the tibia bones fixing in formaldehyde at the moment. We need an effective, quick and cartilage tissue protective decalcification method. We'll perform only light microscopic examination in this study and we have to complete the study within one week. In these circumstances what are your advices?
Thanks in advance.

Dr. Necat Yilmaz
University of Mersin
School of Medicine


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