[Histonet] eosin bleeding on frozen sections

[Laurie Colbert]

Try dehydrating longer.  If there is water left on the slide, the eosin
will bleed.
Laurie Colbert

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Christi
Cosby
Sent: Thursday, April 21, 2011 9:18 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] eosin bleeding on frozen sections

Hi,

In our lab we are having trouble with the eosin bleeding off the section
after coverslipping.  We are performing a rapid H&E stain on frozen
sections.  Any advice would be greatly appreciated!
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