[Histonet] eosin bleeding on frozen sections

[sgoebel <@t> mirnarx.com]

This happened to me a couple weeks ago.  You got water on the slide
after the eosin staining.  Just run it back through alcohols, and rinse
for about five minutes.  Change out the alcohols, and re-stain.

Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Christi
Cosby
Sent: Thursday, April 21, 2011 11:18 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] eosin bleeding on frozen sections

Hi,

In our lab we are having trouble with the eosin bleeding off the section
after coverslipping.  We are performing a rapid H&E stain on frozen
sections.  Any advice would be greatly appreciated!
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