[Histonet] IHC on frozen sections

[Jane C. Moose]

Following is question on behalf of   Jana Moose Ritter, DVM
janamoose <@t> yahoo.com

 

 

We are going to perform IHC on frozen sections, and the tissues  (pig
aorta)  will be harvested on a Friday.

 

Histo lab is wondering:

 

1) can tissue fix in paraformaldehyde over the weekend until next steps
(sucrose and frozen embedding to be completed on Monday) 

2) can fixative be switched to sucrose at the end of Friday (tissue
would remain in sucrose until frozen embedding on Monday)

3) must complete the solution switch and entire frozen embedding process
on Saturday

 

 

Thanks for your help. 

 

Jane Moose

LIS Coordinator

Newberry County Memorial Hospital

Newberry, SC  29108

P-803-405-7129

F- 803-405-7474