[Histonet] IHC on frozen sections
Jane C. Moose
Jane.Moose <@t> NewberryHospital.net
Tue Apr 19 09:18:42 CDT 2011
Following is question on behalf of Jana Moose Ritter, DVM
janamoose <@t> yahoo.com
We are going to perform IHC on frozen sections, and the tissues (pig
aorta) will be harvested on a Friday.
Histo lab is wondering:
1) can tissue fix in paraformaldehyde over the weekend until next steps
(sucrose and frozen embedding to be completed on Monday)
2) can fixative be switched to sucrose at the end of Friday (tissue
would remain in sucrose until frozen embedding on Monday)
3) must complete the solution switch and entire frozen embedding process
on Saturday
Thanks for your help.
Jane Moose
LIS Coordinator
Newberry County Memorial Hospital
Newberry, SC 29108
P-803-405-7129
F- 803-405-7474
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