[Histonet] RE: IHC on frozen sections
Tony Henwood
AnthonyH <@t> chw.edu.au
Tue Apr 19 18:03:23 CDT 2011
Jana,
Since you are fixing in formaldehyde, why not paraffin embed them?
Frozen sectioning of formalin fixed tissue and doing IPX on them is difficult as well as problems with storing the tissue.
I would cut frozen of unfixed tissue and do IPX on them rather than doing IPX on formalin fixed frozen sections.
Any reason why you have chosen the frozen section of formalin fixed tissue?
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jane C. Moose
Sent: Wednesday, 20 April 2011 12:19 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC on frozen sections
Following is question on behalf of Jana Moose Ritter, DVM
janamoose <@t> yahoo.com
We are going to perform IHC on frozen sections, and the tissues (pig
aorta) will be harvested on a Friday.
Histo lab is wondering:
1) can tissue fix in paraformaldehyde over the weekend until next steps (sucrose and frozen embedding to be completed on Monday)
2) can fixative be switched to sucrose at the end of Friday (tissue would remain in sucrose until frozen embedding on Monday)
3) must complete the solution switch and entire frozen embedding process on Saturday
Thanks for your help.
Jane Moose
LIS Coordinator
Newberry County Memorial Hospital
Newberry, SC 29108
P-803-405-7129
F- 803-405-7474
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