[Histonet] RE: IHC on frozen sections

[Tony Henwood]

Jana,

Since you are fixing in formaldehyde, why not paraffin embed them?
Frozen sectioning of formalin fixed tissue and doing IPX on them is difficult as well as problems with storing the tissue.
I would cut frozen of unfixed tissue and do IPX on them rather than doing IPX on formalin fixed frozen sections.

Any reason why you have chosen the frozen section of formalin fixed tissue?

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jane C. Moose
Sent: Wednesday, 20 April 2011 12:19 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC on frozen sections

Following is question on behalf of   Jana Moose Ritter, DVM
janamoose <@t> yahoo.com

 

 

We are going to perform IHC on frozen sections, and the tissues  (pig
aorta)  will be harvested on a Friday.

 

Histo lab is wondering:

 

1) can tissue fix in paraformaldehyde over the weekend until next steps (sucrose and frozen embedding to be completed on Monday) 

2) can fixative be switched to sucrose at the end of Friday (tissue would remain in sucrose until frozen embedding on Monday)

3) must complete the solution switch and entire frozen embedding process on Saturday

 

 

Thanks for your help. 

 

Jane Moose

LIS Coordinator

Newberry County Memorial Hospital

Newberry, SC  29108

P-803-405-7129

F- 803-405-7474

 

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