[Histonet] post-fixation question

[Tony Henwood]

I agree with you Geoff

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Geoff
McAuliffe
Sent: Thursday, 23 September 2010 11:59 PM
To: Salim Yalcin Inan
Cc: Histonet
Subject: Re: [Histonet] post-fixation question


Merced is correct about longer fixation inducing more cross-linking, 
possibly making immunostaining more difficult.
Much depends on the antigen you are looking for.
I disagree that the tissue will become brittle, this is not my
experience.

Geoff


Salim Yalcin Inan wrote:
> Hello,
>
>  
>
> I have a question about post-fixation. As far as I know, for most of 
> the immunohistochemistry studies in mouse/rat brain, post-fixation 
> period is between 2 hours to 24 hours in 4% paraformaldehyde at +4 
> Celsius followed by cryoprotection with 20-30% sucrose solution. Are 
> there any disadvantages to post-fix the tissues for more than 24 hours

> (2 days-1 week) for further immunohistochemistry experiments? Thank 
> you very much in advance.
>
> Sincerely Yours,
>
> Salim Inan, PhD
>
> HBI, University of Calgary
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>   


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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
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