[Histonet] post-fixation question

[Anatoli Gleiberman]

Hi Inan,
2h post-fixation for immunohistochemistry on mouse brain is usually more
than enough. We use 1h post-fixation at room temp for mouse brain
following storage (sometimes, for many weeks) at +4 C in PBS before
sucrose cryo-protection, embedding and sectioning.

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: AGleiberman <@t> cbiolabs.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Salim
Yalcin Inan
Sent: Wednesday, September 22, 2010 5:39 PM
To: Histonet
Subject: [Histonet] post-fixation question

Hello,

 

I have a question about post-fixation. As far as I know, for most of the
immunohistochemistry studies in mouse/rat brain, post-fixation period is
between 2 hours to 24 hours in 4% paraformaldehyde at +4 Celsius
followed by
cryoprotection with 20-30% sucrose solution. Are there any disadvantages
to
post-fix the tissues for more than 24 hours (2 days-1 week) for further
immunohistochemistry experiments? Thank you very much in advance.

Sincerely Yours,

Salim Inan, PhD

HBI, University of Calgary

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