[Histonet] post-fixation question

[Geoff McAuliffe]

Merced is correct about longer fixation inducing more cross-linking, 
possibly making immunostaining more difficult.
Much depends on the antigen you are looking for.
I disagree that the tissue will become brittle, this is not my experience.

Geoff


Salim Yalcin Inan wrote:
> Hello,
>
>  
>
> I have a question about post-fixation. As far as I know, for most of the
> immunohistochemistry studies in mouse/rat brain, post-fixation period is
> between 2 hours to 24 hours in 4% paraformaldehyde at +4 Celsius followed by
> cryoprotection with 20-30% sucrose solution. Are there any disadvantages to
> post-fix the tissues for more than 24 hours (2 days-1 week) for further
> immunohistochemistry experiments? Thank you very much in advance.
>
> Sincerely Yours,
>
> Salim Inan, PhD
>
> HBI, University of Calgary
>
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>
>   


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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
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