[Histonet] Picrosirius Red Staining of mixed archaeological residues. [SEC=UNCLASSIFIED]

[Birgitta Stephenson]

Hello Histonetters,

I have been using Picrosirius Red stain to detect collagen fibers in
residues lifted from archaeological artefacts. The protocol has been
adapted for staining slides rather than tissue samples however I am
curious if it is normal that everything on the slide stains red. I
realise that the collagen fibers can be easily detected in cross
polarised and part polarised light ( yellow and orange = large fibers
and green the thinner fibers and all are highly birefringent).  While
only the collagen changes colours in cross polarised light I am
wondering if it is normal that everything on the slide picks up the
stain to some extent and turns red (pink) when viewed in plane polarised
light? This includes seed parts, plant lipids and ochre? Any thoughts or
ideas?

Thanks 

Regards

Birgitta Stephenson  Archaeological Microscopy Research Lab University
of Queensland

Birgitta Stephenson
Pharmacist Advisor
Veteran Affairs Pharmacy Advisory Centre
Phone (07) 3223 8435
Fax      (07) 3223 8651

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