[Histonet] Help! Curled cryosections

[Teresa Iglesias]

Thanks, Montina
The sections are 40um and I had no trouble with all my other brains
(sectioned myself). I had help with these last two and they are all curled.
I think they just got sectioned too fast or something. I'll try the shaker
and see if it helps.
Thanks!

-Teresa


On Wed, Sep 15, 2010 at 8:47 PM, Montina Van Meter <
Montina.VanMeter <@t> pbrc.edu> wrote:

> Teresa,
> I would put the sections through several 5-10 min. washes on a shaker
> table. It is very important to make sure you have all of the cryoprotectant
> rinsed out of the tissue or it will inhibit IHC staining.  How thick are the
> sections?  Were they cut on a cryostat or freezing microtome?  I routinely
> cut rat brain at 40um and don't have any curling issues.  That sometimes
> occurs when the knife has come through the section of brain too rapidly.  A
> slow and steady motion is needed when cutting frozens.
>
> Good luck!
> Tina Van Meter
>
>
> Sent from my iPhone
>
> On Sep 15, 2010, at 9:37 PM, "Teresa Iglesias" <tliglesias <@t> ucdavis.edu>
> wrote:
>
>
>>


-- 
______________________________
Teresa Iglesias
Graduate Group in Animal Behavior
Department of Evolution and Ecology

University of California-Davis
One Shields Avenue
2320 Storer Hall
Davis, CA 95616
Office: 530-754-7837
Fax: 530-752-1449
tliglesias <@t> ucdavis.edu
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