[Histonet] cryojane problems

Kim Merriam kmerriam2003 <@t> yahoo.com
Wed Oct 27 06:44:28 CDT 2010

Brett- I have been using the 4X slides, but they don't seem to help.  I dont 
have this problem with the cartilage alone, but when there is bone attached, it 
is a nightmare.

Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 

From: "Connolly, Brett M" <brett_connolly <@t> merck.com>
To: Kim Merriam <kmerriam2003 <@t> yahoo.com>; Histonet 
<histonet <@t> lists.utsouthwestern.edu>
Sent: Tue, October 26, 2010 9:41:18 AM
Subject: RE: [Histonet] cryojane problems


I would recommend using their '4x' slides - they have more adhesive on them.

I don't think flashing more than once has any benefit.


Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly <@t> merck.com
T- 215-652-2501
F- 215-993-6803

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Merriam
Sent: Tuesday, October 26, 2010 9:32 AM
To: Histonet
Subject: [Histonet] cryojane problems

Hi All,

I have been using the cryojane to cut human articular cartilage, with excellent 
results.  Now I need to cut frozen sections of rat articular cartilage 
that contain some underlying bone.  I am getting nice sections, but when I peel 
the tape off the slide, a bunch of my tissue is staying on the tape.  I have 
tried increasing the number of flashes (to a whopping 10!), hoping that this 
will help adhere the sections, but it hasn't.

Any tips for me?

 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 

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