Fw: [Histonet] cryojane problems

Candice Smoots candice_camille <@t> yahoo.com
Thu Oct 28 08:48:09 CDT 2010

 I remain yours truely, 

Candice Camille 

----- Forwarded Message ----
From: Candice Smoots <candice_camille <@t> yahoo.com>
To: "Connolly, Brett M" <brett_connolly <@t> merck.com>
Cc: histonet <histonet-request <@t> lists.utsouthwestern.edu>
Sent: Thu, October 28, 2010 8:43:52 AM
Subject: Re: [Histonet] cryojane problems

Hi Guys

I am new to histology. Could you please explain what are flashes?  Thanks

 I remain yours truely, 

Candice Camille 

From: "Connolly, Brett M" <brett_connolly <@t> merck.com>
To: Kim Merriam <kmerriam2003 <@t> yahoo.com>; Histonet 
<histonet <@t> lists.utsouthwestern.edu>
Sent: Tue, October 26, 2010 8:41:18 AM
Subject: RE: [Histonet] cryojane problems


I would recommend using their '4x' slides - they have more adhesive on them.

I don't think flashing more than once has any benefit.


Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly <@t> merck.com
T- 215-652-2501
F- 215-993-6803

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Merriam
Sent: Tuesday, October 26, 2010 9:32 AM
To: Histonet
Subject: [Histonet] cryojane  problems

Hi All,

I have been using the cryojane to cut human articular cartilage, with excellent 
results.  Now I need to cut frozen sections of rat articular cartilage 
that contain some underlying bone.  I am getting nice sections, but when I peel 
the tape off the slide, a bunch of my tissue is staying on the tape.  I have 
tried increasing the number of flashes (to a whopping 10!), hoping that this 
will help adhere the sections, but it hasn't.

Any tips for me?

 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu


More information about the Histonet mailing list