[Histonet] cryojane problems

[Connolly, Brett M]

Kim,

I would recommend using their '4x' slides - they have more adhesive on them.

I don't think flashing more than once has any benefit.

Brett

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly <@t> merck.com
T- 215-652-2501
F- 215-993-6803


 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Merriam
Sent: Tuesday, October 26, 2010 9:32 AM
To: Histonet
Subject: [Histonet] cryojane problems

Hi All,

I have been using the cryojane to cut human articular cartilage, with excellent 
results.  Now I need to cut frozen sections of rat articular cartilage 
that contain some underlying bone.  I am getting nice sections, but when I peel 
the tape off the slide, a bunch of my tissue is staying on the tape.  I have 
tried increasing the number of flashes (to a whopping 10!), hoping that this 
will help adhere the sections, but it hasn't.

Any tips for me?

Kim 
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 


      
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