[Histonet] eosin in processor

[Patrick Laurie]

We don't, Leica recommends that you don't.  We have our grossing group
squirt a 0.1% Basic Fuchsin on the small translucent or white specimens.  It
leaves it light pink like the eosin.

On Fri, Oct 22, 2010 at 11:09 AM, Rathborne, Toni <
trathborne <@t> somerset-healthcare.com> wrote:

> Does anyone use eosin in their Peloris?
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Shea's
> Sent: Friday, October 22, 2010 2:07 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] eosin in processor
>
>
> This is the best kept secret....For those who have never tried this, you
> don't know what you are missing in the ease of embedding and cutting.
>
> We have been adding alcoholic eosin (about 10cc each) to the 1st & 2nd 100%
> alcohols, leaving the last 100% pure (of course there is carry over during
> the week), then we dump the first and rotate the other 2 each week. The one
> that we now moved into position #2 will get 10cc eosin).
>
> We send our IHC & FISH  to a HUGE reference lab. I specifically asked about
> this interfering and the technical rep said that it is not a problem esp
> they see a lot of it and they know to avoid non specific perimeter staining
> (the Pathologists know how to read around this because many labs are using
> Eosin in the processors).
>
> J
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-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plaurie <@t> cellnetix.com