[Histonet] eosin

Cathy cfitz <@t> 007group.com
Fri Oct 22 10:46:33 CDT 2010

We add 1.0 mls of 10% aqueous Eosin to the first 100% Ethanol; we will then
have Eosin in all of the Ethanol containers after they have been rotated.
This removed the time consuming task of dotting each tissue at grossing and
allows for easy visualization at embedding and sectioning.  I have not had a
complaint about the eosin interfering with FISH from our referral lab.


Cathy Fitzpatrick

Anatomic Pathology Section Head

Kelowna General Hospital




From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histotech <@t> imagesbyhopper.com
Sent: Friday, October 22, 2010 8:18 AM
To: Tench, Bill
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] eosin


It sounds like I am in the minority in pitting the eosin in the *first* 100%

I agree with Bill, it really makes a HUGE difference in small, minute
tissues.  We did run into one issue though, we were using orange biopsy
cassettes and the red-orange tissue was difficult to spot in the orange
cassettes!  We have since switched to an aqua color and no more hiding
specimens!  ;o)


On Oct 22, 2010, at 10:51 AM, "Tench, Bill" <Bill.Tench <@t> pph.org> wrote:

> I think the use in a processor becomes a local culture.  I think
> everyone in my community does it (someone spread the word), so now we
> all do.  I believe that we are all putting it in the last alcohol.  It
> really makes facing a block on minute pieces a whole lot easier.  We
> found marking the tissue directly tedious, and it didn't persist as well
> in the processing.
>  Because there is often so little visible material in our FNA cell
> blocks, we mark the histogel pellet with Davidson ink, and when the tech
> has just faced off the ink, he/she knows its time to start collecting
> sections.
> Bill Tench
> Associate Dir. Laboratory Services
> Chief, Cytology Services
> Palomar Medical Center
> 555 E. Valley Parkway
> Escondido, California  92025
> Bill.Tench <@t> pph.org
> Voice: 760- 739-3037
> Fax: 760-739-2604
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