[Histonet] eosin

Blazek, Linda lblazek <@t> digestivespecialists.com
Fri Oct 22 10:22:51 CDT 2010

We put eosin in the first 100% alcohol.  

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histotech <@t> imagesbyhopper.com
Sent: Friday, October 22, 2010 11:18 AM
To: Tench, Bill
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] eosin

It sounds like I am in the minority in pitting the eosin in the *first* 100% alcohol!  

I agree with Bill, it really makes a HUGE difference in small, minute tissues.  We did run into one issue though, we were using orange biopsy cassettes and the red-orange tissue was difficult to spot in the orange cassettes!  We have since switched to an aqua color and no more hiding specimens!  ;o)


On Oct 22, 2010, at 10:51 AM, "Tench, Bill" <Bill.Tench <@t> pph.org> wrote:

> I think the use in a processor becomes a local culture.  I think
> everyone in my community does it (someone spread the word), so now we
> all do.  I believe that we are all putting it in the last alcohol.  It
> really makes facing a block on minute pieces a whole lot easier.  We
> found marking the tissue directly tedious, and it didn't persist as well
> in the processing.
>  Because there is often so little visible material in our FNA cell
> blocks, we mark the histogel pellet with Davidson ink, and when the tech
> has just faced off the ink, he/she knows its time to start collecting
> sections.
> Bill Tench
> Associate Dir. Laboratory Services
> Chief, Cytology Services
> Palomar Medical Center
> 555 E. Valley Parkway
> Escondido, California  92025
> Bill.Tench <@t> pph.org
> Voice: 760- 739-3037
> Fax: 760-739-2604
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