[Histonet] Endothelial histochemistry

[Kristen Lauing]

Hi Ali,
Although I do not ever perform endothelial immunohistochemistry in our laboratory, I know it is common to use an antibody against CD31 to selectively stain endothelial cells and this may work well in pituitary tissue.
Hope that helps,
Kristen


>>> Ali Nasr Esfahani <alina211 <@t> student.liu.se> 10/22/10 5:31 AM >>>
Hi, I am just new in this group, so I hope this is the way I can get help.

I am doing histochemistry on human hypothalamus and I need to stain
endothelial cells. I tried UEA-I (lectin) and I got microvessels stained as
the whole and like a line. But I would like to have an image of the
endothelial cells of even larger vessels, the way that I can show cell by
cell in a circular shape of a vessel.

Could you help me and tell me what antibody or lectin helps me best.

Regards

Ali
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