[Histonet] negative controls

[histotech <@t> imagesbyhopper.com]

I run both positive and negative controls.  Wherever possible, I put the
positive control on the same slide as the test patient (this makes it easier
for the pathologist and it also saves us the cost of on slide being tested).
I always run a negative patient control on the exact same run.  If I have
more slides than will fit on one run, I will hold the specific case back, as
I won't separate them.  Matters not if there is more than one machine, the
case will stay together.

As far as negatives, I will run an extra negative if my run has both poly
and mono antibodies.  So, there are many times, I have two negatives running
per case.  I run only one detection system, so I don't have that variable to
deal with.

Michelle



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kuhnla,
Melissa
Sent: Friday, October 15, 2010 12:50 PM
To: Victoria Baker; Histo Net list server
Subject: RE: [Histonet] negative controls


Hi Vikki,
I have 1 Ventana XT and 3 Ultras.  I have certain antibodies designated to
each ultra.  This means not all slides from any case are guaranteed to be on
the same machine.  They obviously all have their own detection kit.  My
theory is that our detection kits are QCd prior to initial load onto an
instrument. The patient block was treated the same prior to cutting. The
slides receive solutions that are QCd...I think we are covered. What are
your thoughts? Melissa
  

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Victoria
Baker
Sent: Friday, October 15, 2010 10:26 AM
To: Histo Net list server
Subject: [Histonet] negative controls

Hi
I have a hypothetical question to those who run IHC on Ventana instruments.
Are you running your negatives with your patient/test cases or on a separate
run?  Also, if you are doing this and have to use a different detection kit
how do you work the QA/QC portion of this for CAP requirements.

Thanks

Vikki
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