[Histonet] I have a couple of quick questions

[Rene J Buesa]

Yes, xylene is the way to go.
No, 37ºC will not hurt the sections no matter for how long they are at that temp.
René J.

--- On Thu, 10/14/10, Lewis, Patrick <patrick.lewis <@t> seattlechildrens.org> wrote:


From: Lewis, Patrick <patrick.lewis <@t> seattlechildrens.org>
Subject: [Histonet] I have a couple of quick questions
To: Histonet <@t> lists.utsouthwestern.edu
Date: Thursday, October 14, 2010, 7:11 PM


If I want to remove a cover slip that is coversliped with permount.  Do
I need to use xylene to get rid of the semi hardened permount gunk?



Also,  If I am heating paraffin sectioned slides overnight at 37C to
adhere them what happens if I over cook them by leaving them at 37C for
more than one day?



Say 3-4 days at 37C  It probably wont hurt them as they haven't been
epitope retrieved yet.















Patrick Lewis

Research Associate II-Bench| Infections and Prematurity

Seattle Children's Research Institute

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