[Histonet] I have a couple of quick questions

[BSullivan <@t> shorememorial.org]

Yes..... to remove mounting media you need to leave them in the Xylene
after the coverslip is removed. If the mounting media is not totally
removed your stain, whatever one you are doing, will not give you the
results you need.

Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590


                                                                           
             "Lewis, Patrick"                                              
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             ern.edu                   [Histonet] I have a couple of quick 
                                       questions                           
                                                                           
             10/14/2010 07:11                                              
             PM                                                            
                                                                           
                                                                           
                                                                           




If I want to remove a cover slip that is coversliped with permount.  Do
I need to use xylene to get rid of the semi hardened permount gunk?



Also,  If I am heating paraffin sectioned slides overnight at 37C to
adhere them what happens if I over cook them by leaving them at 37C for
more than one day?



Say 3-4 days at 37C  It probably wont hurt them as they haven't been
epitope retrieved yet.















Patrick Lewis

Research Associate II-Bench| Infections and Prematurity

Seattle Children's Research Institute

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patrick.lewis <@t> seattlechildrens.org

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