Re: [Histonet] I have a couple of quick questions

[godsgalnow@aol.com]

Yes, soak slides in xylene to remove coverslip.  

A few days in 37 degree oven won't hurt

Roxanne

Sent from my Verizon Wireless Phone

----- Reply message -----
From: "Lewis, Patrick" <patrick.lewis <@t> seattlechildrens.org>
Date: Thu, Oct 14, 2010 7:11 pm
Subject: [Histonet] I have a couple of quick questions
To: <Histonet <@t> lists.utsouthwestern.edu>

If I want to remove a cover slip that is coversliped with permount.  Do
I need to use xylene to get rid of the semi hardened permount gunk?

 

Also,  If I am heating paraffin sectioned slides overnight at 37C to
adhere them what happens if I over cook them by leaving them at 37C for
more than one day?

 

Say 3-4 days at 37C  It probably wont hurt them as they haven't been
epitope retrieved yet.

 

 

 

 

 

 

 

Patrick Lewis

Research Associate II-Bench| Infections and Prematurity

Seattle Children's Research Institute

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patrick.lewis <@t> seattlechildrens.org

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WWW     seattlechildrens.org <http://seattlechildrens.org/> 

 



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