[Histonet] I have a couple of quick questions

Lewis, Patrick patrick.lewis <@t> seattlechildrens.org
Thu Oct 14 18:11:59 CDT 2010


If I want to remove a cover slip that is coversliped with permount.  Do
I need to use xylene to get rid of the semi hardened permount gunk?

 

Also,  If I am heating paraffin sectioned slides overnight at 37C to
adhere them what happens if I over cook them by leaving them at 37C for
more than one day?

 

Say 3-4 days at 37C  It probably wont hurt them as they haven't been
epitope retrieved yet.

 

 

 

 

 

 

 

Patrick Lewis

Research Associate II-Bench| Infections and Prematurity

Seattle Children's Research Institute

206-884-1115  OFFICE 

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206-884-7311  FAX

patrick.lewis <@t> seattlechildrens.org

OFFICE  1900 9th Avenue Seattle, WA 98101

MAIL      M/S C9S-8, Seattle, WA 98101

WWW     seattlechildrens.org <http://seattlechildrens.org/> 

 



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