[Histonet] RE: New Cap Guidelines for Her2 and ER/PR

Wed Oct 13 12:51:45 CDT 2010

Fixation prior to the processor will not exce4ed 12 hrs.  That is why we
program the processor for 36...not to exceed 48.

________________________________

From: Mike Pence [mailto:mpence <@t> grhs.net] 
Sent: Wednesday, October 13, 2010 11:04 AM
To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR

How much time is the tissue in formalin prior to going in the processor?
Your total time in formalin can not exceed 48 hr. And you will still
need to validate your process if you hold your tissue longer than
"normal processing" time in 70% (ie. more than 1 hr).

	-----Original Message-----
	From: Kuhnla, Melissa [mailto:Melissa.Kuhnla <@t> chsli.org] 
	Sent: Wednesday, October 13, 2010 9:45 AM
	To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline;
histonet <@t> lists.utsouthwestern.edu
	Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
ER/PR

	For the weekend, we have our processor set for 36 hours in
formalin and then a hold in 70%. This allows for complete fixation and
cuts down on prolonged time in 70%

________________________________

	From: Phyllis Thaxton [mailto:dchihc <@t> yahoo.com] 
	Sent: Wednesday, October 13, 2010 10:32 AM
	To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline;
histonet <@t> lists.utsouthwestern.edu
	Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and
ER/PR

	 We run a weekend (Friday til Monday AM)  breast run where the
tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in
order to complete processing on Monday morning. So far no problems.

	Phyllis Thaxton HT(ASCP)QIHC
	DCH Regional Medical Center
	Tuscaloosa, AL 

________________________________

	From: "Kuhnla, Melissa" <Melissa.Kuhnla <@t> chsli.org>
	To: "Mahoney,Janice A" <Janice.Mahoney <@t> alegent.org>; Mike Pence
<mpence <@t> grhs.net>; Joyce Cline <Joyce.Cline <@t> wchsys.org>;
histonet <@t> lists.utsouthwestern.edu
	Sent: Tue, October 12, 2010 12:02:32 PM
	Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
ER/PR

	I disagree.  Prolonged formalin fixation (over 48 hrs),
diminishes
	signals

	-----Original Message-----
	From: histonet-bounces <@t> lists.utsouthwestern.edu
	[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
	Mahoney,Janice A
	Sent: Tuesday, October 12, 2010 12:05 PM
	To: 'Mike Pence'; Joyce Cline; histonet <@t> lists.utsouthwestern.edu
	Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
ER/PR

	Formalin fixation time does not impact the results of FISH as it
does
	IHC.
	Jan M
	Omaha

	-----Original Message-----
	From: Mike Pence [mailto:mpence <@t> grhs.net]
	Sent: Tuesday, October 12, 2010 11:00 AM
	To: Mahoney,Janice A; Joyce Cline;
histonet <@t> lists.utsouthwestern.edu
	Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
ER/PR

	I don't think it matters if you do Her2 by FISH or IHC the time
is still
	48hr. I hope I am wrong, but I don't think I am.

	Mike

	-----Original Message-----
	From: histonet-bounces <@t> lists.utsouthwestern.edu
	[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
	Mahoney,Janice A
	Sent: Tuesday, October 12, 2010 10:25 AM
	To: 'Joyce Cline'; histonet <@t> lists.utsouthwestern.edu
	Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR

	We have decided to reflex to FISH those breasts that do not fall
within
	the recommended formalin fixation time.  We do work on Saturdays
so it
	is only the rare 3 day weekends that this comes into play. Jan M
Omaha

	-----Original Message-----
	From: histonet-bounces <@t> lists.utsouthwestern.edu
	[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Joyce
	Cline
	Sent: Tuesday, October 12, 2010 10:10 AM
	To: histonet <@t> lists.utsouthwestern.edu
	Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR

	Does anyone have any experience with storing formalin fixed
breast
	tissue in 70% before processing?  I am trying to comply with the
new
	guidelines set forth by CAP and ASCO with regard to Her2 and
ER/PR and
	since my lab does not operate on the weekend we have been well
above the
	48 hour recommended formalin fixation time.  Does 70% affect
	antigenicity for either Her2 or ER/PR?  Any information or
suggestions
	will be greatly appreciated.  Thanks :)

	Ronda Souders
	Hagerstown Medical Laboratory
	301-665-4980
	fax 301-665-4941
	ronda.souders <@t> wchsys.org<mailto:ronda.souders <@t> wchsys.org>

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