[Histonet] RE: New Cap Guidelines for Her2 and ER/PR
Mike Pence
mpence <@t> grhs.net
Wed Oct 13 10:04:04 CDT 2010
How much time is the tissue in formalin prior to going in the processor?
Your total time in formalin can not exceed 48 hr. And you will still
need to validate your process if you hold your tissue longer than
"normal processing" time in 70% (ie. more than 1 hr).
-----Original Message-----
From: Kuhnla, Melissa [mailto:Melissa.Kuhnla <@t> chsli.org]
Sent: Wednesday, October 13, 2010 9:45 AM
To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
ER/PR
For the weekend, we have our processor set for 36 hours in
formalin and then a hold in 70%. This allows for complete fixation and
cuts down on prolonged time in 70%
________________________________
From: Phyllis Thaxton [mailto:dchihc <@t> yahoo.com]
Sent: Wednesday, October 13, 2010 10:32 AM
To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline;
histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and
ER/PR
We run a weekend (Friday til Monday AM) breast run where the
tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in
order to complete processing on Monday morning. So far no problems.
Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL
________________________________
From: "Kuhnla, Melissa" <Melissa.Kuhnla <@t> chsli.org>
To: "Mahoney,Janice A" <Janice.Mahoney <@t> alegent.org>; Mike Pence
<mpence <@t> grhs.net>; Joyce Cline <Joyce.Cline <@t> wchsys.org>;
histonet <@t> lists.utsouthwestern.edu
Sent: Tue, October 12, 2010 12:02:32 PM
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
ER/PR
I disagree. Prolonged formalin fixation (over 48 hrs),
diminishes
signals
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Mahoney,Janice A
Sent: Tuesday, October 12, 2010 12:05 PM
To: 'Mike Pence'; Joyce Cline; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
ER/PR
Formalin fixation time does not impact the results of FISH as it
does
IHC.
Jan M
Omaha
-----Original Message-----
From: Mike Pence [mailto:mpence <@t> grhs.net]
Sent: Tuesday, October 12, 2010 11:00 AM
To: Mahoney,Janice A; Joyce Cline;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
ER/PR
I don't think it matters if you do Her2 by FISH or IHC the time
is still
48hr. I hope I am wrong, but I don't think I am.
Mike
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Mahoney,Janice A
Sent: Tuesday, October 12, 2010 10:25 AM
To: 'Joyce Cline'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
We have decided to reflex to FISH those breasts that do not fall
within
the recommended formalin fixation time. We do work on Saturdays
so it
is only the rare 3 day weekends that this comes into play. Jan M
Omaha
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Joyce
Cline
Sent: Tuesday, October 12, 2010 10:10 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR
Does anyone have any experience with storing formalin fixed
breast
tissue in 70% before processing? I am trying to comply with the
new
guidelines set forth by CAP and ASCO with regard to Her2 and
ER/PR and
since my lab does not operate on the weekend we have been well
above the
48 hour recommended formalin fixation time. Does 70% affect
antigenicity for either Her2 or ER/PR? Any information or
suggestions
will be greatly appreciated. Thanks :)
Ronda Souders
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
ronda.souders <@t> wchsys.org<mailto:ronda.souders <@t> wchsys.org>
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