[Histonet] RE: New Cap Guidelines for Her2 and ER/PR

[Bill B.]

How do you define fixation time? A half pound hunk of fat with a tumor in the middle will remain unfixed until blocked. A small biopsy will start fixing almost immediately. 

Bill

At 1:51 PM -0400 10/13/10, Kuhnla, Melissa wrote:
>Fixation prior to the processor will not exce4ed 12 hrs.  That is why we
>program the processor for 36...not to exceed 48.
>
> 
>
>________________________________
>
>From: Mike Pence [mailto:mpence <@t> grhs.net] 
>Sent: Wednesday, October 13, 2010 11:04 AM
>To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline;
>histonet <@t> lists.utsouthwestern.edu
>Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
>
> 
>
>How much time is the tissue in formalin prior to going in the processor?
>Your total time in formalin can not exceed 48 hr. And you will still
>need to validate your process if you hold your tissue longer than
>"normal processing" time in 70% (ie. more than 1 hr).
>
>	-----Original Message-----
>	From: Kuhnla, Melissa [mailto:Melissa.Kuhnla <@t> chsli.org] 
>	Sent: Wednesday, October 13, 2010 9:45 AM
>	To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline;
>histonet <@t> lists.utsouthwestern.edu
>	Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
>ER/PR
>
>	For the weekend, we have our processor set for 36 hours in
>formalin and then a hold in 70%. This allows for complete fixation and
>cuts down on prolonged time in 70%
>
>	 
>
>	
>________________________________
>
>
>	From: Phyllis Thaxton [mailto:dchihc <@t> yahoo.com] 
>	Sent: Wednesday, October 13, 2010 10:32 AM
>	To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline;
>histonet <@t> lists.utsouthwestern.edu
>	Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and
>ER/PR
>
>	 
>
>	
>	 We run a weekend (Friday til Monday AM)  breast run where the
>tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in
>order to complete processing on Monday morning. So far no problems.
>
>	 
>
>	Phyllis Thaxton HT(ASCP)QIHC
>	DCH Regional Medical Center
>	Tuscaloosa, AL 
>
>	 
>
>	 
>
>	
>________________________________
>
>
>	From: "Kuhnla, Melissa" <Melissa.Kuhnla <@t> chsli.org>
>	To: "Mahoney,Janice A" <Janice.Mahoney <@t> alegent.org>; Mike Pence
><mpence <@t> grhs.net>; Joyce Cline <Joyce.Cline <@t> wchsys.org>;
>histonet <@t> lists.utsouthwestern.edu
>	Sent: Tue, October 12, 2010 12:02:32 PM
>	Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
>ER/PR
>	
>	I disagree.  Prolonged formalin fixation (over 48 hrs),
>diminishes
>	signals
>	
>	-----Original Message-----
>	From: histonet-bounces <@t> lists.utsouthwestern.edu
>	[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
>	Mahoney,Janice A
>	Sent: Tuesday, October 12, 2010 12:05 PM
>	To: 'Mike Pence'; Joyce Cline; histonet <@t> lists.utsouthwestern.edu
>	Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
>ER/PR
>	
>	Formalin fixation time does not impact the results of FISH as it
>does
>	IHC.
>	Jan M
>	Omaha
>	
>	-----Original Message-----
>	From: Mike Pence [mailto:mpence <@t> grhs.net]
>	Sent: Tuesday, October 12, 2010 11:00 AM
>	To: Mahoney,Janice A; Joyce Cline;
>histonet <@t> lists.utsouthwestern.edu
>	Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
>ER/PR
>	
>	I don't think it matters if you do Her2 by FISH or IHC the time
>is still
>	48hr. I hope I am wrong, but I don't think I am.
>	
>	Mike
>	
>	-----Original Message-----
>	From: histonet-bounces <@t> lists.utsouthwestern.edu
>	[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
>	Mahoney,Janice A
>	Sent: Tuesday, October 12, 2010 10:25 AM
>	To: 'Joyce Cline'; histonet <@t> lists.utsouthwestern.edu
>	Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
>	
>	
>	We have decided to reflex to FISH those breasts that do not fall
>within
>	the recommended formalin fixation time.  We do work on Saturdays
>so it
>	is only the rare 3 day weekends that this comes into play. Jan M
>Omaha
>	
>	-----Original Message-----
>	From: histonet-bounces <@t> lists.utsouthwestern.edu
>	[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
>Joyce
>	Cline
>	Sent: Tuesday, October 12, 2010 10:10 AM
>	To: histonet <@t> lists.utsouthwestern.edu
>	Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR
>	
>	Does anyone have any experience with storing formalin fixed
>breast
>	tissue in 70% before processing?  I am trying to comply with the
>new
>	guidelines set forth by CAP and ASCO with regard to Her2 and
>ER/PR and
>	since my lab does not operate on the weekend we have been well
>above the
>	48 hour recommended formalin fixation time.  Does 70% affect
>	antigenicity for either Her2 or ER/PR?  Any information or
>suggestions
>	will be greatly appreciated.  Thanks :)
>	
>	
>	Ronda Souders
>	Hagerstown Medical Laboratory
>	301-665-4980
>	fax 301-665-4941
>	ronda.souders <@t> wchsys.org<mailto:ronda.souders <@t> wchsys.org>