[Histonet] RE: New Cap Guidelines for Her2 and ER/PR

[Phyllis Thaxton]

Rene,
 8 hours is what is on the processor. We keep a log book on all breast tissue. 
The OR nurse documents on the label;  the time of excision (placed in 10% NBF) . 
The gross assistant documents the time of dissection, Then the time the tissues 
are placed on the processor. Our fixation times for breast average about 12 to 
14 hours. Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL 




________________________________
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
To: MelissaKuhnla <Melissa.Kuhnla <@t> chsli.org>; Janice AMahoney 
<Janice.Mahoney <@t> alegent.org>; Mike Pence <mpence <@t> grhs.net>; Joyce Cline 
<Joyce.Cline <@t> wchsys.org>; histonet <@t> lists.utsouthwestern.edu; Phyllis Thaxton 
<dchihc <@t> yahoo.com>
Sent: Wed, October 13, 2010 9:39:21 AM
Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR


Phyllis:
The time required in NBF is not 8 hours only, that is exactly what the 
regulations are trying to avoid, i.e. incomplete fixation.
René J.

--- On Wed, 10/13/10, Phyllis Thaxton <dchihc <@t> yahoo.com> wrote:


>From: Phyllis Thaxton <dchihc <@t> yahoo.com>
>Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
>To: "Kuhnla, Melissa" <Melissa.Kuhnla <@t> chsli.org>, "Mahoney,Janice A" 
><Janice.Mahoney <@t> alegent.org>, "Mike Pence" <mpence <@t> grhs.net>, "Joyce Cline" 
><Joyce.Cline <@t> wchsys.org>, histonet <@t> lists.utsouthwestern.edu
>Date: Wednesday, October 13, 2010, 10:32 AM
>
>
>
> We run a weekend (Friday til Monday AM)  breast run where the tissues are in 
>10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete 
>processing on Monday morning. So far no problems.
>Phyllis Thaxton HT(ASCP)QIHC
>DCH Regional Medical Center
>Tuscaloosa, AL 
>
>
>
>
>________________________________
>From: "Kuhnla, Melissa" <Melissa.Kuhnla <@t> chsli.org>
>To: "Mahoney,Janice A" <Janice.Mahoney <@t> alegent.org>; Mike Pence 
><mpence <@t> grhs.net>; Joyce Cline <Joyce.Cline <@t> wchsys.org>; 
>histonet <@t> lists.utsouthwestern.edu
>Sent: Tue, October 12, 2010 12:02:32 PM
>Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
>
>I disagree.  Prolonged formalin fixation (over 48 hrs), diminishes
>signals
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
>Mahoney,Janice A
>Sent: Tuesday, October 12, 2010 12:05 PM
>To: 'Mike Pence'; Joyce Cline; histonet <@t> lists.utsouthwestern.edu
>Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
>
>Formalin fixation time does not impact the results of FISH as it does
>IHC.
>Jan M
>Omaha
>
>-----Original Message-----
>From: Mike Pence [mailto:mpence <@t> grhs.net]
>Sent: Tuesday, October 12, 2010 11:00 AM
>To: Mahoney,Janice A; Joyce Cline; histonet <@t> lists.utsouthwestern.edu
>Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
>
>I don't think it matters if you do Her2 by FISH or IHC the time is still
>48hr. I hope I am wrong, but I don't think I am.
>
>Mike
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
>Mahoney,Janice A
>Sent: Tuesday, October 12, 2010 10:25 AM
>To: 'Joyce Cline'; histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
>
>
>We have decided to reflex to FISH those breasts that do not fall within
>the recommended formalin fixation time.  We do work on Saturdays so it
>is only the rare 3 day weekends that this comes into play. Jan M Omaha
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joyce
>Cline
>Sent: Tuesday, October 12, 2010 10:10 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR
>
>Does anyone have any experience with storing formalin fixed breast
>tissue in 70% before processing?  I am trying to comply with the new
>guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and
>since my lab does not operate on the weekend we have been well above the
>48 hour recommended formalin fixation time.  Does 70% affect
>antigenicity for either Her2 or ER/PR?  Any information or suggestions
>will be greatly appreciated.  Thanks :)
>
>
>Ronda Souders
>Hagerstown Medical Laboratory
>301-665-4980
>fax 301-665-4941
>ronda.souders <@t> wchsys.org<mailto:ronda.souders <@t> wchsys.org>
>
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