[Histonet] RE: New Cap Guidelines for Her2 and ER/PR

[Tench, Bill]

 Have you validated this processing?  Leaving the tissue in 70% alcohol for 48 hours is not standard, and thus requires all of the hassles associated with validation.


Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench <@t> pph.org
Voice: 760- 739-3037
Fax: 760-739-2604

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Phyllis Thaxton
Sent: Wednesday, October 13, 2010 7:32 AM
To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR


 We run a weekend (Friday til Monday AM)  breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems.
Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL 




________________________________
From: "Kuhnla, Melissa" <Melissa.Kuhnla <@t> chsli.org>
To: "Mahoney,Janice A" <Janice.Mahoney <@t> alegent.org>; Mike Pence <mpence <@t> grhs.net>; Joyce Cline <Joyce.Cline <@t> wchsys.org>; histonet <@t> lists.utsouthwestern.edu
Sent: Tue, October 12, 2010 12:02:32 PM
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR

I disagree.  Prolonged formalin fixation (over 48 hrs), diminishes signals

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A
Sent: Tuesday, October 12, 2010 12:05 PM
To: 'Mike Pence'; Joyce Cline; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR

Formalin fixation time does not impact the results of FISH as it does IHC.
Jan M
Omaha

-----Original Message-----
From: Mike Pence [mailto:mpence <@t> grhs.net]
Sent: Tuesday, October 12, 2010 11:00 AM
To: Mahoney,Janice A; Joyce Cline; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR

I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am.

Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A
Sent: Tuesday, October 12, 2010 10:25 AM
To: 'Joyce Cline'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR


We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time.  We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joyce Cline
Sent: Tuesday, October 12, 2010 10:10 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR

Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing?  I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the
48 hour recommended formalin fixation time.  Does 70% affect antigenicity for either Her2 or ER/PR?  Any information or suggestions will be greatly appreciated.  Thanks :)


Ronda Souders
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
ronda.souders <@t> wchsys.org<mailto:ronda.souders <@t> wchsys.org>

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