[Histonet] RE: New Cap Guidelines for Her2 and ER/PR

[Rene J Buesa]

Phyllis:
The time required in NBF is not 8 hours only, that is exactly what the regulations are trying to avoid, i.e. incomplete fixation.
René J.

--- On Wed, 10/13/10, Phyllis Thaxton <dchihc <@t> yahoo.com> wrote:


From: Phyllis Thaxton <dchihc <@t> yahoo.com>
Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
To: "Kuhnla, Melissa" <Melissa.Kuhnla <@t> chsli.org>, "Mahoney,Janice A" <Janice.Mahoney <@t> alegent.org>, "Mike Pence" <mpence <@t> grhs.net>, "Joyce Cline" <Joyce.Cline <@t> wchsys.org>, histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, October 13, 2010, 10:32 AM



 We run a weekend (Friday til Monday AM)  breast run where the tissues are in 
10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete 
processing on Monday morning. So far no problems.
Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL 




________________________________
From: "Kuhnla, Melissa" <Melissa.Kuhnla <@t> chsli.org>
To: "Mahoney,Janice A" <Janice.Mahoney <@t> alegent.org>; Mike Pence 
<mpence <@t> grhs.net>; Joyce Cline <Joyce.Cline <@t> wchsys.org>; 
histonet <@t> lists.utsouthwestern.edu
Sent: Tue, October 12, 2010 12:02:32 PM
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR

I disagree.  Prolonged formalin fixation (over 48 hrs), diminishes
signals

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Mahoney,Janice A
Sent: Tuesday, October 12, 2010 12:05 PM
To: 'Mike Pence'; Joyce Cline; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR

Formalin fixation time does not impact the results of FISH as it does
IHC.
Jan M
Omaha

-----Original Message-----
From: Mike Pence [mailto:mpence <@t> grhs.net]
Sent: Tuesday, October 12, 2010 11:00 AM
To: Mahoney,Janice A; Joyce Cline; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR

I don't think it matters if you do Her2 by FISH or IHC the time is still
48hr. I hope I am wrong, but I don't think I am.

Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Mahoney,Janice A
Sent: Tuesday, October 12, 2010 10:25 AM
To: 'Joyce Cline'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR


We have decided to reflex to FISH those breasts that do not fall within
the recommended formalin fixation time.  We do work on Saturdays so it
is only the rare 3 day weekends that this comes into play. Jan M Omaha

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joyce
Cline
Sent: Tuesday, October 12, 2010 10:10 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR

Does anyone have any experience with storing formalin fixed breast
tissue in 70% before processing?  I am trying to comply with the new
guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and
since my lab does not operate on the weekend we have been well above the
48 hour recommended formalin fixation time.  Does 70% affect
antigenicity for either Her2 or ER/PR?  Any information or suggestions
will be greatly appreciated.  Thanks :)


Ronda Souders
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
ronda.souders <@t> wchsys.org<mailto:ronda.souders <@t> wchsys.org>

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