[Histonet] problems with staining IHC

[Cathy]

We and several other sites (in British Columbia and Alberta) that I have
contacted have been experiencing IHC staining problems.  Ours started in
August while others have been seeing this since May.  We have had conference
calls with Ventana about this and they have said that it is a problem with
the SuperFrost Plus slides; the slides seem to be hydrophobic.  The staining
that we are seeing is negative controls/positive patient, positive
control/negative patient as well as variable counterstain.  We have control
tissue on every slide.  From what we have learned from Fisher and Ventana
they are working together with Erie Scientific (manufacturer of the Plus
slides) to find out what is causing the problem.  The slides appear to be
vary from box to box and within each box.  Some lot numbers are worse than
others; our most recent lot number was 062510-9.  I would suggest that you
contact your sale person as well as the platform vendor (if your staining is
automated) so they are aware of how large the problem may be.  We have not
found another slide that is any better than the Plus.  Ventana is working on
an official communication for their clients.  We are very frustrated with
the lack of progress in solving the problem.

As anyone noticed that the Plus slides don't seem to have the same adhesive
properties that they used to?

Cathy Fitzpatrick
Anatomic Pathology Section Head
Kelowna General Hospital
Kelowna, B.C.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
srishan <@t> mail.holyname.org
Sent: Thursday, October 07, 2010 7:32 AM
To: histonet-bounces <@t> lists.utsouthwestern.edu;
Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] problems with staining IHC

Hi All,

Our pathologists have made a complain that the staining of slides have 
been a problem.  Same antibodies which stain one day does not work the 
following day.  This has been going on for a few months.  When we repeat 
stain they either work or not work and we have been sending them to 
reference labs. 

Our tech support person was here and told us that the slides ( fisher plus 
slides) have been a problem with some of her customers.  Meanwhile our 
surrounding labs which are using the same slides have no issues.  She 
showed us how the reagents were not spreading properly on the slides. 

Is anyone confronted this issue?  If so,  how was it solved? 

Thanks in advance.

Mala Srishan
Supervisor, Histology
Holy Name Medical Center
Teaneck NJ 07666


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