[Histonet] problems with staining IHC

[Esparza, Sandra]

If Erie is the manufacture of your Fisher slides then yes we have had
the same problem with our IHC's.  We use the Ventana Benchmark and have
had this problem off and on for about 8 months.  There seems to be no
consistency with the quality of slides from Erie.  We have just switched
to the TruBond 200 slides which are distributed by STATLABS.  These are
working great for our IHC's.

Sandra
Sandra Esparza HT(ASCP)QIHC
Lead Technologist
Dell Children's Medical Center of Central Texas
512-324-0000  x87061
sesparza <@t> seton.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tim
Higgins
Sent: Thursday, October 07, 2010 10:53 AM
To: srishan <@t> mail.holyname.org;
histonet-bounces <@t> lists.utsouthwestern.edu;
Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] problems with staining IHC

Hi Mala,

We've used the Fisher superfrost plus slides for years and have never
had
that issue, it sounds more like you need to add some Tween to your
buffer.
That will help the solutions spread along your slide.

Hope that helps. 


Thanks,
 
Timothy Higgins, HT(ASCP) QIHC

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
srishan <@t> mail.holyname.org
Sent: Thursday, October 07, 2010 9:32 AM
To: histonet-bounces <@t> lists.utsouthwestern.edu;
Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] problems with staining IHC

Hi All,

Our pathologists have made a complain that the staining of slides have
been
a problem.  Same antibodies which stain one day does not work the
following
day.  This has been going on for a few months.  When we repeat stain
they
either work or not work and we have been sending them to reference labs.


Our tech support person was here and told us that the slides ( fisher
plus
slides) have been a problem with some of her customers.  Meanwhile our
surrounding labs which are using the same slides have no issues.  She
showed
us how the reagents were not spreading properly on the slides. 

Is anyone confronted this issue?  If so,  how was it solved? 

Thanks in advance.

Mala Srishan
Supervisor, Histology
Holy Name Medical Center
Teaneck NJ 07666


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