[Histonet] problems with staining IHC

[Tim Higgins]

Hi Mala,

We've used the Fisher superfrost plus slides for years and have never had
that issue, it sounds more like you need to add some Tween to your buffer.
That will help the solutions spread along your slide.

Hope that helps. 


Thanks,
 
Timothy Higgins, HT(ASCP) QIHC

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
srishan <@t> mail.holyname.org
Sent: Thursday, October 07, 2010 9:32 AM
To: histonet-bounces <@t> lists.utsouthwestern.edu;
Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] problems with staining IHC

Hi All,

Our pathologists have made a complain that the staining of slides have been
a problem.  Same antibodies which stain one day does not work the following
day.  This has been going on for a few months.  When we repeat stain they
either work or not work and we have been sending them to reference labs. 

Our tech support person was here and told us that the slides ( fisher plus
slides) have been a problem with some of her customers.  Meanwhile our
surrounding labs which are using the same slides have no issues.  She showed
us how the reagents were not spreading properly on the slides. 

Is anyone confronted this issue?  If so,  how was it solved? 

Thanks in advance.

Mala Srishan
Supervisor, Histology
Holy Name Medical Center
Teaneck NJ 07666


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