[Histonet] problems with staining IHC

[Jan Shivers]

Mala,

If your main problem is that the reagents aren't spreading across your 
slides, I agree that you should put Tween 20 (0.05% per volume) in all of 
your rinse buffers between steps, regardless of if you do your IHC stains 
manually or with an autostainer.

If this doesn't solve the problem, post your method on histonet so that we 
know more about your procedure steps and can send additional advice.

Jan Shivers
UMN Vet Diag Lab

----- Original Message ----- 
From: <srishan <@t> mail.holyname.org>
To: <histonet-bounces <@t> lists.utsouthwestern.edu>; 
<Histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, October 07, 2010 9:32 AM
Subject: [Histonet] problems with staining IHC


> Hi All,
>
> Our pathologists have made a complain that the staining of slides have
> been a problem.  Same antibodies which stain one day does not work the
> following day.  This has been going on for a few months.  When we repeat
> stain they either work or not work and we have been sending them to
> reference labs.
>
> Our tech support person was here and told us that the slides ( fisher plus
> slides) have been a problem with some of her customers.  Meanwhile our
> surrounding labs which are using the same slides have no issues.  She
> showed us how the reagents were not spreading properly on the slides.
>
> Is anyone confronted this issue?  If so,  how was it solved?
>
> Thanks in advance.
>
> Mala Srishan
> Supervisor, Histology
> Holy Name Medical Center
> Teaneck NJ 07666
>
>
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