[Histonet] problems with staining IHC

Sebree Linda A LSebree <@t> uwhealth.org
Thu Oct 7 09:55:58 CDT 2010


What kind of stainers are you using and is it happening on all your
stainers?  Or are you staining by hand? 


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
srishan <@t> mail.holyname.org
Sent: Thursday, October 07, 2010 9:32 AM
To: histonet-bounces <@t> lists.utsouthwestern.edu;
Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] problems with staining IHC

Hi All,

Our pathologists have made a complain that the staining of slides have 
been a problem.  Same antibodies which stain one day does not work the 
following day.  This has been going on for a few months.  When we repeat

stain they either work or not work and we have been sending them to 
reference labs. 

Our tech support person was here and told us that the slides ( fisher
plus 
slides) have been a problem with some of her customers.  Meanwhile our 
surrounding labs which are using the same slides have no issues.  She 
showed us how the reagents were not spreading properly on the slides. 

Is anyone confronted this issue?  If so,  how was it solved? 

Thanks in advance.

Mala Srishan
Supervisor, Histology
Holy Name Medical Center
Teaneck NJ 07666


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet








Holy Name Medical Center is the recipient of:

Magnet Recognition for Excellence in Patient Care, American Nurses 
Credentialing Center

100 Best Places to Work in Healthcare, Ranked Fourth Nationally by
Modern 
Healthcare

Best Places to Work in New Jersey, NJBIZ

Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. 
Power

Distinguished Hospital Awards for Clinical Excellence, HealthGrades

Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, 
HealthGrades

Best in Value Award, Data Advantage, LLC

Chest Pain Center Accreditation, Society of Chest Pain Centers

Primary Stroke Center Designation, The Joint Commission and NJ
Department 
of Health and Human Services


**** Warning: The information contained in this message is privileged
and 
CONFIDENTIAL and is intended only for the use of the addressee above. If

you are not the intended recipient, you are hereby notified that any 
disclosure, copying, distribution, or taking of any action in reliance
on 
the content of this message is strictly prohibited. If you have received

this communication in error, please notify the sender by replying to
this 
message, and then delete it from your system.




_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



More information about the Histonet mailing list