[Histonet] problems with staining IHC

[Blazek, Linda]

Hi Mala,
I had this same problem.  It was solved by adding Tween to the buffer.  It made the reagents flow much better over the slides.  Also before you stain, put the slides in a rinse of buffer/tween and agitate them for about 30 seconds.

Linda


Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 396-2623
Email: lblazek <@t> digestivespecialists.com



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of srishan <@t> mail.holyname.org
Sent: Thursday, October 07, 2010 10:32 AM
To: histonet-bounces <@t> lists.utsouthwestern.edu; Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] problems with staining IHC

Hi All,

Our pathologists have made a complain that the staining of slides have 
been a problem.  Same antibodies which stain one day does not work the 
following day.  This has been going on for a few months.  When we repeat 
stain they either work or not work and we have been sending them to 
reference labs. 

Our tech support person was here and told us that the slides ( fisher plus 
slides) have been a problem with some of her customers.  Meanwhile our 
surrounding labs which are using the same slides have no issues.  She 
showed us how the reagents were not spreading properly on the slides. 

Is anyone confronted this issue?  If so,  how was it solved? 

Thanks in advance.

Mala Srishan
Supervisor, Histology
Holy Name Medical Center
Teaneck NJ 07666


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