[Histonet] PAS STAIN

[Geoff McAuliffe]

I make my Schiff's from scratch and store it in the refrigerator.
I use a minimum amount of charcoal and I heat it in the oven at over 100 
C the night before to be sure it is nice and dry.
I make periodic acid fresh that day.
I make bisulfite rinses fresh that day.
Aqueous formalin for 48 hours at room temp. is OK for rat and mouse 
liver glycogen, I don't know about other species. Slices of liver should 
be thin.
When in doubt formalin+alcohol+acetic acid is an excellent fixative.

Geoff


Diana McCaig wrote:
> Hi
> We have been doing a PAS stain on the same control block and same
> reagent supplier for a long time.  
> Yesterday when we ran the slides, they failed to stain
>  
> We opened new bottles of Periodic Acid and Schiff's Reagent and recut
> fresh controls
>  
> Still, we are unable to get any staining.
>  
> Suggestions to help would be appreciated
>  
> Diana
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
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