[Histonet] AChE fiber staining protocoll?

Houston, Ronald Ronald.Houston <@t> nationwidechildrens.org
Wed Oct 6 08:50:07 CDT 2010

I have had great success in fine fibers with the modification of Martucciello et al, Eur J Pediatr Surg 2001; 11: 300-304, but only in 15 micron intestine sections, so I do not know how it will transpose to brain studies and the thicker sections

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of szigcs <@t> bio.u-szeged.hu
Sent: Wednesday, October 06, 2010 3:34 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] AChE fiber staining protocoll?

Dear Histonet Members!

We are going to investigate AChE fiber density changes in the cortex  
of transcardially perfused rat brain slices (30 micrometer, criostat  
sections, perfusing solution: 4% formaldehyde in PB ph 7.4). We need a  
protocoll to visualize FINE fiber structure to be able to count changes.

We  used modification of Hedreen and original Tago protocoll. Hedreen  
good results for general, but no fiber staining, Tago no sucsses.

Could you please tell us some advise, protocolls functioning on teh  
above mentioned objects?

Thank you all in advance...
Csaba Szigeti

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