[Histonet] AChE fiber staining protocoll?

[szigcs <@t> bio.u-szeged.hu]

IdĂŠzet ("Houston, Ronald" <Ronald.Houston <@t> nationwidechildrens.org>):


Thank you for the answer. We will try this protocol and i will reply  
the results.
Csaba Szigeti

> I have had great success in fine fibers with the modification of   
> Martucciello et al, Eur J Pediatr Surg 2001; 11: 300-304, but only   
> in 15 micron intestine sections, so I do not know how it will   
> transpose to brain studies and the thicker sections
>
> Ronnie Houston
> Anatomic Pathology Manager
> Nationwide Children's Hospital
> Columbus OH 43205
> (614) 722 5450
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu   
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of   
> szigcs <@t> bio.u-szeged.hu
> Sent: Wednesday, October 06, 2010 3:34 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] AChE fiber staining protocoll?
>
> Dear Histonet Members!
>
> We are going to investigate AChE fiber density changes in the cortex
> of transcardially perfused rat brain slices (30 micrometer, criostat
> sections, perfusing solution: 4% formaldehyde in PB ph 7.4). We need a
> protocoll to visualize FINE fiber structure to be able to count changes.
>
> We  used modification of Hedreen and original Tago protocoll. Hedreen
> good results for general, but no fiber staining, Tago no sucsses.
>
> Could you please tell us some advise, protocolls functioning on teh
> above mentioned objects?
>
> Thank you all in advance...
> Csaba Szigeti
>
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