[Histonet] HMGB and RAGE; TLR2, TLR4 antibodies

[Amos Brooks]

Hi,
     I am currently working on HMGB1. It is a weird one! If you go by the
data sheets you will get great nuclear staining on all the nuclei. This is
not what you are after if you have necrotic tissue. What you want to see is
the nuclei in necrotic tissue fade out and have the cytoplasm take it up. I
am cutting the dilution to 1: 50 and 1:100 since we were just starting to
see blushes of cytoplasmic staining and no nuclear label at higher
dilutions. Usually you get staining that fades out at higher dilutions. This
one was really odd.

Good luck,
Amos


Message: 13
Date: Thu, 30 Sep 2010 04:55:02 +0200
From: Fabrice GANKAM <gankam <@t> googlemail.com>
Subject: [Histonet] HMGB and RAGE; TLR2, TLR4 antibodies
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C4A46C631CD14A86AAD3CE3ABB29A7DF <@t> PCdeGANKAM>
Content-Type: text/plain;       charset="us-ascii"


Hey guys
Just wanted to know if any of you had some luck with an antibody aigainst
rat HMGB1 and its receptors RAGE, TLR2, TLR4.
W
Thanks
Dr Fabrice GANKAM
UTSW