[Histonet] HMGB and RAGE; TLR2, TLR4 antibodies

[Fabrice GANKAM]

thanks Amos
which one did you used ? you have catalog number ?

On Fri, Oct 1, 2010 at 9:44 PM, Amos Brooks <amosbrooks <@t> gmail.com> wrote:

> Hi,
>      I am currently working on HMGB1. It is a weird one! If you go by the
> data sheets you will get great nuclear staining on all the nuclei. This is
> not what you are after if you have necrotic tissue. What you want to see is
> the nuclei in necrotic tissue fade out and have the cytoplasm take it up. I
> am cutting the dilution to 1: 50 and 1:100 since we were just starting to
> see blushes of cytoplasmic staining and no nuclear label at higher
> dilutions. Usually you get staining that fades out at higher dilutions. This
> one was really odd.
>
> Good luck,
> Amos
>
>
> Message: 13
> Date: Thu, 30 Sep 2010 04:55:02 +0200
> From: Fabrice GANKAM <gankam <@t> googlemail.com>
> Subject: [Histonet] HMGB and RAGE; TLR2, TLR4 antibodies
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <C4A46C631CD14A86AAD3CE3ABB29A7DF <@t> PCdeGANKAM>
> Content-Type: text/plain;       charset="us-ascii"
>
>
> Hey guys
> Just wanted to know if any of you had some luck with an antibody aigainst
> rat HMGB1 and its receptors RAGE, TLR2, TLR4.
> W
> Thanks
> Dr Fabrice GANKAM
> UTSW
>