SPAM-LOW: [Histonet] shrinkage during IHC

Patsy Ruegg pruegg <@t>
Sun Nov 7 14:20:58 CST 2010

You should have the same morphology from the IHC slides done outside that
you get from your H&E, do you provide them with slides or the Block?  The
only thing I can think of is if they airdry after IHC instead of going thru
alcohols and xylene to coverslip (if they use AEC or ap/red they may
airdry), I have seen cell shrinkage from airdrying after IHC occasionally.
Something else just occurred to me, if they over heat during HIER I suppose
this could happen, but I have not seen it.



Patsy Ruegg, HT(ASCP)QIHC
12635 Montview Blvd. Ste.215
Aurora, CO 80045
fax 720-859-4110

-----Original Message-----
From: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] On Behalf Of
zodiac29 <@t>
Sent: Saturday, November 06, 2010 6:28 AM
To: histonet <@t>
Subject: SPAM-LOW: [Histonet] shrinkage during IHC

To All, 

We are a lab that sends our specimens out for IHC and have just switched to
another reference laboratory for these services. Our pathologist is saying
that the tissue looks shrunk on the IHC slides, yet the slides that I
process (H&E, and special stains)are fine. Does anyone know what is causing
this? The reference lab said it could be the type of slides that I use to
mount the sections we send to them. My knowledge in IHC is limited. Also, if
this helps, they are FFPE tissue. 	

Thanks for your help


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