[Histonet] RE: eosin as tissue dye

[McMahon, Loralee A]

We have not had any problem with fluorescence with our her 2 FISH or any complaints from the Cytogenetics labs which run many other types of FISH.  We use eosin in our processors.   The eosin will bleed out when you deparaffinize the slides.  


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa [Melissa.Kuhnla <@t> chsli.org]
Sent: Thursday, November 04, 2010 9:16 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] eosin as tissue dye

I know using eosin as a tissue dye during processing has been a recent
discussion.  My lab has been experiencing issue with the cobalt blue we
use currently.  At embedding, small biopsies are not seen on blue
sponges, white sponges turn blue. Biopsies that are wrapped are not
turning blue, the paper is not allowing the blue thru.  Eosin has come
up as a suggestion.  I know it is known to fluoresce. What is everyone's
experience with this??  The only FISH test we are running is Her2 by
pathvysion.  What recipe are you all using?? On the processor??
Thank you so much.
Melissa


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