[Histonet] unstained paraffin tissue slides storage--why cold?

Sebree Linda A LSebree <@t> uwhealth.org
Thu Nov 4 09:40:05 CDT 2010

Some antigens degrade and lose antigenicity in paraffin sections when
stored at RT.  Storage at below freezing temps slows this process down .

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Emily
Sent: Thursday, November 04, 2010 9:08 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] unstained paraffin tissue slides storage--why

Can I ask what the point of storing paraffin sections in freezing cold
They are wax sections, which never see any type of cold, so I don't
understand the point of this.  I do understand putting them at 4 degrees
prevent mold, but -80 seems excessive.
We have kept our slides at room temperature for years and years, but
slides do not have an albumin coat (which I can see getting moldy), just
chemical coating.
Fixing for paraffin and paraffin infiltration seems to keep antigens
without refrigeration because it's so intense, but that's just
conjecture on
my part.

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