[Histonet] unstained paraffin tissue slides storage--why cold?

sgoebel <@t> xbiotech.com sgoebel <@t> xbiotech.com
Thu Nov 4 10:21:03 CDT 2010

-70 or -80 seems a little extreme to me, that's why I always just leave
them in a normal freezer (-20).  I think the main point of doing this
from what I understand is so that the antigens stay "viable".  I know
over time they can degrade and so your stain won't work with some
antibodies.  The weirdest part to me has always been that you don't have
to store the blocks this way.  So I think that was your question, if the
blocks aren't stored in a freezer why store the slides?  Won't the
antigens in the blocks start to degrade as well?  This is a question I
would like to know the answer to as well...

Sarah Goebel, B.A., HT (ASCP)

XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas  78744


-------- Original Message --------
Subject: Re: [Histonet] unstained paraffin tissue slides storage--why
From: Emily Sours <talulahgosh <@t> gmail.com>
Date: Thu, November 04, 2010 7:07 am
To: histonet <@t> lists.utsouthwestern.edu

Can I ask what the point of storing paraffin sections in freezing cold
They are wax sections, which never see any type of cold, so I don't
understand the point of this. I do understand putting them at 4 degrees
prevent mold, but -80 seems excessive.
We have kept our slides at room temperature for years and years, but
slides do not have an albumin coat (which I can see getting moldy), just
chemical coating.
Fixing for paraffin and paraffin infiltration seems to keep antigens
without refrigeration because it's so intense, but that's just
conjecture on
my part.

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