[Histonet] AlphaSMA staining

[Mark Tarango]

Hi Phebe,

I can't be sure about this since you didn't post your protocol, but
alpha-SMA is an antibody that does not require antigen retrieval.  If you're
doing some kind of retrieval, I'd suggest trying it without.

Thanks

Mark

On Wed, May 26, 2010 at 6:56 PM, Phebe Verbrugghe <vphebe4 <@t> yahoo.com> wrote:

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> Hello everyone,
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> We are trying to stain alpha smooth muscle actin (on mouse/human liver)
> using the A5228 Sigma antibody and are getting nuclear staining while this
> stain should be cytoplasmic. Does anyone have experience with nuclear
> staining of alphaSMA? Is there anything I can do to avoid nuclear staining
> or can anyone recommend me another good anti- alphaSMA antibody that works
> on both mouse and human formalin fixed paraffin sections?
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> Thank you very much in advance!
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> Phebe
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