SPAM-LOW: Re: [Histonet] AlphaSMA staining

[Patsy Ruegg]

Phebe,

It does sound like you are over heating or digesting so I agree with Mark
try this without any antigen retrieval.

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mark Tarango
Sent: Thursday, May 27, 2010 8:30 AM
To: Phebe Verbrugghe
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: Re: [Histonet] AlphaSMA staining

Hi Phebe,

I can't be sure about this since you didn't post your protocol, but
alpha-SMA is an antibody that does not require antigen retrieval.  If you're
doing some kind of retrieval, I'd suggest trying it without.

Thanks

Mark

On Wed, May 26, 2010 at 6:56 PM, Phebe Verbrugghe <vphebe4 <@t> yahoo.com> wrote:

>
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> Hello everyone,
>
> We are trying to stain alpha smooth muscle actin (on mouse/human liver)
> using the A5228 Sigma antibody and are getting nuclear staining while this
> stain should be cytoplasmic. Does anyone have experience with nuclear
> staining of alphaSMA? Is there anything I can do to avoid nuclear staining
> or can anyone recommend me another good anti- alphaSMA antibody that works
> on both mouse and human formalin fixed paraffin sections?
>
> Thank you very much in advance!
>
> Phebe
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