[Histonet] AlphaSMA staining

[Phebe Verbrugghe]

Hello everyone,
 
We are trying to stain alpha smooth muscle actin (on mouse/human liver) using the A5228 Sigma antibody and are getting nuclear staining while this stain should be cytoplasmic. Does anyone have experience with nuclear staining of alphaSMA? Is there anything I can do to avoid nuclear staining or can anyone recommend me another good anti- alphaSMA antibody that works on both mouse and human formalin fixed paraffin sections?
 
Thank you very much in advance!
 
Phebe