[Histonet] weekend fixation

[Mark Tarango]

I just realized the question came from Belgium.  I have no idea how they do
things there.

Mark

On Thu, May 20, 2010 at 2:14 PM, Mark Tarango <marktarango <@t> gmail.com> wrote:

> Yes, you can go up to 72 hours for ER/PR (new CAP/ASCO guidlines), but if
> you do HER2 the maximum is still 48 hours.  I'm assuming you want HER2 as
> well, so your best option would probably be to hold the tissues in 70%
> alcochol on the processer until Sunday night.
>
> Mark Tarango
>   On Thu, May 20, 2010 at 12:54 PM, Liz Chlipala <liz <@t> premierlab.com>wrote:
>
>> I was talking to Peggy Wenk over the weekend at the MSH meeting and they
>> had a paper that was published regarding fixation and ER/PR staining
>> sensitivity etc.  The biggest problem that they reported is
>> underfixation is much worse than over fixation.  I think a minimum of 10
>> hours of fixation demonstrated good results and that intensity of
>> staining started to decrease but not by much at 48 hours.  I would be
>> more concerned over underfixation than overfixation.  Also the new ER/PR
>> guidelines state its acceptable to have samples in fixative for 72
>> hours.  Maybe Peggy can post the link to this paper.
>>
>> Liz
>>
>> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
>> Manager
>> Premier Laboratory, LLC
>> PO Box 18592
>> Boulder, Colorado 80308
>> office (303) 682-3949
>> fax (303) 682-9060
>> www.premierlab.com
>>
>>
>> Ship to Address:
>> 1567 Skyway Drive, Unit E
>> Longmont, Colorado 80504
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of LINDA
>> MARGRAF
>> Sent: Thursday, May 20, 2010 1:04 PM
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] weekend fixation
>>
>>
>>   Histonetters:
>> Here's a message I was asked to post......
>>
>> Dear Colleagues,
>>  I  have the following question concerning tissue processing. We do a
>> lot of IHC work on NF fixed tissue. To standardize and minimize the
>> effect of NF fixation, we fixate the tissue always for 24h. This is of
>> course a problem for tissues taken on Friday. In the past, we asked our
>> technicians to come on Saturday to embed the tissues in paraffin.
>> Unfortunately, this is not possible anymore, and that is why I need your
>> advice. What would you suggest ? 1) to leave the tissue in NF until
>> Sunday evening and start processing, or 2) to keep the fixation time (24
>> hours) and leave the tissue in alcohol 70% until Sunday evening and then
>> start processing.
>>  Thanks for your advice.
>> Kind regards,
>> Wim.
>>
>>
>> Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
>> Cell biology and Histology
>> Department of Morphology - Faculty of Veterinary Medicine
>> Ghent University
>> Salisburylaan 133, B-9820 Merelbeke, BELGIUM
>>
>>
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