[Histonet] weekend fixation

[Mike Pence]

The problem with all this is that the ER/PR fixation times and the her2
fixation times do not match 72 hrs vs 48 hrs. Many times the same
specimen that receives ER/PR also gets her2. This is where things are
missed up. Why would they ever change one and not the other?!@#$%^&*

Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Liz
Chlipala
Sent: Thursday, May 20, 2010 2:54 PM
To: LINDA MARGRAF; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] weekend fixation


I was talking to Peggy Wenk over the weekend at the MSH meeting and they
had a paper that was published regarding fixation and ER/PR staining
sensitivity etc.  The biggest problem that they reported is
underfixation is much worse than over fixation.  I think a minimum of 10
hours of fixation demonstrated good results and that intensity of
staining started to decrease but not by much at 48 hours.  I would be
more concerned over underfixation than overfixation.  Also the new ER/PR
guidelines state its acceptable to have samples in fixative for 72
hours.  Maybe Peggy can post the link to this paper.  

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of LINDA
MARGRAF
Sent: Thursday, May 20, 2010 1:04 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] weekend fixation


 Histonetters:
Here's a message I was asked to post......

Dear Colleagues,
 I  have the following question concerning tissue processing. We do a
lot of IHC work on NF fixed tissue. To standardize and minimize the
effect of NF fixation, we fixate the tissue always for 24h. This is of
course a problem for tissues taken on Friday. In the past, we asked our
technicians to come on Saturday to embed the tissues in paraffin.
Unfortunately, this is not possible anymore, and that is why I need your
advice. What would you suggest ? 1) to leave the tissue in NF until
Sunday evening and start processing, or 2) to keep the fixation time (24
hours) and leave the tissue in alcohol 70% until Sunday evening and then
start processing.  Thanks for your advice. Kind regards, Wim.
 

Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
Cell biology and Histology
Department of Morphology - Faculty of Veterinary Medicine
Ghent University
Salisburylaan 133, B-9820 Merelbeke, BELGIUM



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