[Histonet] weekend fixation

[Mark Tarango]

Yes, you can go up to 72 hours for ER/PR (new CAP/ASCO guidlines), but if
you do HER2 the maximum is still 48 hours.  I'm assuming you want HER2 as
well, so your best option would probably be to hold the tissues in 70%
alcochol on the processer until Sunday night.

Mark Tarango
On Thu, May 20, 2010 at 12:54 PM, Liz Chlipala <liz <@t> premierlab.com> wrote:

> I was talking to Peggy Wenk over the weekend at the MSH meeting and they
> had a paper that was published regarding fixation and ER/PR staining
> sensitivity etc.  The biggest problem that they reported is
> underfixation is much worse than over fixation.  I think a minimum of 10
> hours of fixation demonstrated good results and that intensity of
> staining started to decrease but not by much at 48 hours.  I would be
> more concerned over underfixation than overfixation.  Also the new ER/PR
> guidelines state its acceptable to have samples in fixative for 72
> hours.  Maybe Peggy can post the link to this paper.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Manager
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, Colorado 80308
> office (303) 682-3949
> fax (303) 682-9060
> www.premierlab.com
>
>
> Ship to Address:
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>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of LINDA
> MARGRAF
> Sent: Thursday, May 20, 2010 1:04 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] weekend fixation
>
>
>   Histonetters:
> Here's a message I was asked to post......
>
> Dear Colleagues,
>  I  have the following question concerning tissue processing. We do a
> lot of IHC work on NF fixed tissue. To standardize and minimize the
> effect of NF fixation, we fixate the tissue always for 24h. This is of
> course a problem for tissues taken on Friday. In the past, we asked our
> technicians to come on Saturday to embed the tissues in paraffin.
> Unfortunately, this is not possible anymore, and that is why I need your
> advice. What would you suggest ? 1) to leave the tissue in NF until
> Sunday evening and start processing, or 2) to keep the fixation time (24
> hours) and leave the tissue in alcohol 70% until Sunday evening and then
> start processing.
>  Thanks for your advice.
> Kind regards,
> Wim.
>
>
> Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
> Cell biology and Histology
> Department of Morphology - Faculty of Veterinary Medicine
> Ghent University
> Salisburylaan 133, B-9820 Merelbeke, BELGIUM
>
>
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